novel variants of citrus exocortis viroid from southern of iran

نویسندگان

saa bagherian

department of horticultural sciences, college of agriculture, jahrom university, jahrom, iran

چکیده

background and aims: citrus exocortis disease is naturally limited to citrus plants and causes a serious disease on sensitive rootstocks such as trifoliate orange. the aim of this study was to sample new variants of citrus exocortis viroid from fars trees with yellowing and suberization symptoms and comparison with other isolates of this viroid. materials and methods: a number of symptomatic or symptomless citrus trees were sampled and studied for viroid detection. after rna extraction, reverse transcription-polymerase chain reaction was conducted and the full length genome of viroids were cloned and sequenced. three cevd isolates from fars were selected and used for comparison with four cevd isolates from mazandaran and a number of other sequences from genbank. results: it was shown that on the basis of homology, three cevd isolates from mazandaran and all three cevd isolates from fars clustered into one group while one of the mazandaran sequences fell apart. conclusions: therefore we proposed that iranian cevd isolates except one isolate from north have the same origin.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Novel variants of citrus exocortis viroid from southern of Iran

Background and Aims: Citrus exocortis disease is naturally limited to citrus plants and causes a serious disease on sensitive rootstocks such as trifoliate orange. The aim of this study was to sample new variants of citrus exocortis viroid from Fars trees with yellowing and suberization symptoms and comparison with other isolates of this viroid. Materials and Methods: A number of symptomat...

متن کامل

Host-directed processing of Citrus exocortis viroid.

Prolonged infection of tomato hybrid (Lycopersicon esculentum x Lycopersicon peruvianum) by Citrus exocortis viroid (CEVd) resulted in viroid-like enlarged structures, detected by gel electrophoresis. This population included two new enlarged variants or D-variants, D-87 and D-76, and three transient species or D-forms, D-38, D-40 and D-43. Sequence analyses exposed a locus near the terminal re...

متن کامل

ogenicity, viroid evolution, Citrus exocortis viroid, Potato spindle tuber viroid, Peach latent mosaic viroid, Chrysan- themum chlorotic mottle viroid

Viroids are autonomously replicating, small singlestranded circular RNA molecules that do not code for proteins and may cause disease in infected, susceptible plants. Viroids have the ability to induce both RNA-mediated transcriptional gene silencing (TGS) and posttranscriptional gene silencing (PTGS) in infected plants. Viroid PTGS has also been demonstrated in a wheat germ extract system. A p...

متن کامل

Citrus Bent Leaf Viroid from Fars Province of Iran

Background and Aims: CBLVd has been reported from northern of Iran previously. The aim of this study was evaluating new viroid variant from asymptomatic citrus trees of Fars province and comparing with its other isolates. Materials and Methods: In this study a number of citrus trees without symptoms were sampled and subjected to RNA extraction followed by RT-PCR, cloning and sequencing of ...

متن کامل

Isolates of citrus exocortis viroid recovered by host and tissue selection.

Isolates of citrus exocortis viroid (CEV) from a single sweet orange citrus source have been selected by sequential passage through the alternative hosts citron, Gynura aurantiaca, a hybrid tomato Lycopersicon esculentum x L. peruvianum, and from disorganized callus culture of the hybrid tomato. The distinctions in symptom expression, titre and electrophoretic mobility among the CEV isolates, o...

متن کامل

Accumulation of Viroid RNA in Tumor Cells After Double Infection by Agrobacterium tumefaciens and Citrus Exocortis Viroid

SEMANCIK, J. S., L. K. GRILL, and E. L. CIVEROLO. 1978. Accumulation of viroid RNA in tumor cells after double infection by Agrobacterium tumefaciens and citrus exocortis viroid. Phytopathology 68: 1288-1292. The presence of citrus exocortis viroid (CEV) in tumors RNase-resistant ' I-CEV following molecular hybridization induced by A. tumefaciens in CEV-infected tomato has been of nucleic acid ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of virology

جلد ۹، شماره ۳، صفحات ۲۴-۳۱

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023